Objective To study the Acaroid mites breeding in Chinese herbal medicine Fructificatio Auricularia auricular and describe the morphology of Lepidoglyphus destructor. Methods The Chinese herbal medicine Fructificatio Auricularia auricular was collected from pharmacy and hospital Chinese medicinal materials warehouse in Wuhu city in July 2015, vibration sieve was used to separate the mites, and the morphological observation was conducted for the mite specites Lepidoglyphus destructor. Results The detection rate of acaroids mite was 76.67%, with the average breeding density of 2.09/g, total 9 kinds of acaroids mites were collected. The eggs, larvae, protonymph, dormancy, tritonymph and adult mites of Lepidoglyphus destructor were visible in Chinese medicinal materials Fructificatio Auricularia auricular. Conclusion The Acaroid mites breed seriously in Chinese herbal medicine Fructificatio Auricularia auricular, and need to adopt measures for prevention and control of the mite breeding.

Objective To study the structure and feeding habits of Neoseiulus barkeri. Methods After oscillating and washing, the mite was killed with heated alcohol, then placed in the preservation solution. Mounting medium zishi was applied to the slide in the amount of 2-3 drops, and a cover glass was placed after adjusted mite posture with hair needle. The specimens were dried for identification under light microscope. Results Length of N. barkeri male body is about 310-350 μm, with light gray or light brown in color. Corniculus is long and slender, aggregated to center. The fixed digit, which has three tines, and movable digit, which has one denticulation, are approximately equal in length. The spermatodactyl is shap of "T". There are 17 pair setae on the back, all equal length except Z₅ which with comb shape and slightly longer. Outer edge hair r₃ and R₁ are located on the back. Besides anal seta there have other 4 pair setae. The female body length is about 370-390 μm. Color and shape is similar to those in male. The chelicerae is bigger than male's but the anal plate is smaller than male's. The egg is colorless and transparent, with oval shape, and size of (80-85) μm×(60-65) μm. Each female mite preyed Caloglyphus berlesei at an average of (12.80±1.47) per day and C. berlesei eggs with an average of (17.70±1.86) per day. When prey was absence, there was a tendency of self-mutilation. Conclusion We gained certain understanding of the morphology and feeding of N. barkeri, which provide a foundation for the further study.

Objective To investigate the breeding of the mite Tarsonemus granarius in the stored Chinese herb safflower. Methods Samples of Carthamus tinctorius were collected from Wuhu, Huainan and Xuancheng in China. The mites T. granarius were isolated with directicopy and waternacopy, and identified and counted under the light microscope. Results The mites T. granarius were found in the stored Chinese herb safflower. The breeding rate and breeding density was 6.50% (13/200) and 6.12 heads/g (796 heads/130 g), respectively. The composition of adult mites accounted for 85.80%, young mites 12.82% and eggs 1.38%. Conclusion Our findings suggest that the mite T. granarius breed in the stored Chinese herb safflower, and this condition requires our attention to prevent and control.

Objective To investigate the species and ecological differences of mite breeding in wheat bran stored in different ways. Methods Twenty-five wheat bran samples stored in different ways (bulk, package, and bucket) were collected from northern Anhui province, China. Mites breeding in the samples were isolated, identified, and counted. The following ecological indices were analyzed: breeding density, richness index, diversity index, and evenness index. Differences in the rate of mite breeding between the three storage methods were examined using 2 test. Results Eight species of mites were identified from 75 wheat bran samples, mainly including Caloglyphus berlesei (40.7% ) and Tyrophagus putrescentiae (33.3%). The breeding densities of mites in bulk, package, and bucket storage were 0.50, 0.25, and 0.30 mite/g, respectively. The corresponding values of richness index were 1.59, 1.18, and 0.94, respectively;the values of diversity index were 1.536, 1.222, and 1.167, respectively;the values of evenness index were 0.739, 0.759, and 0.725, respectively. The rates of mite breeding in bulk, package, and bucket storage were 48.0%, 24.0%, and 16.0%, respectively, with significant differences between the three storage methods ( χ²=6.047, P<0.05). Conclusion Mite breeding exists in wheat bran storage in northern Anhui province, where the rate of mite breeding is affected by the way of storage.

Objective To compare the quality between the glass specimens of acaroid mites prepared with three mounting media. Methods Polyethylene lactic acid phenol medium, Berlese medium, and Hoyer's medium were prepared according to formulae, and their physical and chemical properties were compared. Sixty glass specimens of acaroid mites were prepared with each medium and were observed under a optical microscope after being oven-dried. The obtained data were analyzed by chi-square test. Results Polyethylene lactic acid phenol medium was light yellow liquid, with a density of 0.99 g/ml. Berlese medium was reddish brown, with a density of 1.12 g/ml. Hoyer's medium was slightly lighter than Berlese medium, with a density of 1.37 g/ml. The optical microscopy showed that the glass specimens of acaroid mites prepared with Hoyer's medium had high transparency and stretched mite bodies, with little yellowing and blackening at both sides of mite bodies; the specimens prepared with the other two media had relatively low quality. The chi-square test revealed significant differences between the three media (χ²=15.54, P<0.01), and the specimens prepared with Hoyer's medium had significantly higher quality than that prepared with polyethylene lactic acid phenol medium (χ²=7.50, P<0.01) and that prepared with Berlese medium (χ²=15.70, P<0.01). Conclusion Glass specimens of acaroid mites prepared with Hoyer's medium have the best quality under a optical microscope, providing a reference for the preparation of high-quality glass specimens of acaroid mites.

Objective To investigate the breeding of acaroid mites in room air conditioners in Wuhu, China. Methods Dust was collected from the dust screens of the cabinet air conditioners and wall-mounted air conditioners used by Wuhu residents from June to October 2012. The mites breeding in the dust were classified and identified, and the mites of each species were counted. The mite breeding rates were compared by chi-square test. Results A total of 202 dust samples were collected from the dust screens of air conditioners; 3265 mites, including 2796 acaroid mites, were detected from the dust samples, and they were identified as 18 species, 14 genera, and 6 families. The mean breeding density was 10.39 mites/g of dust, and the breeding rate was 70.79%. The mite breeding rate for the cabinet air conditioners was 58.73%, versus 76.26% for the wall-mounted air conditioners (χ²=6.442, P<0.05). Pyroglyphidae, Acaridae, and Glycyphagidae were commonly seen and accounted for 53.47%, 20.96%, and 18.13% of the total tmites, respectively. Conclusion In Wuhu, acaroid mites breed heavily in the dust screens of cabinet air conditioners and wall-mounted air conditioners, with high species diversity.

Objective To study the community structure and diversity of mites breeding in tobacco storage in Anhui province, China. Methods Samples (tobacco leaves and tobacco dust on the ground) were obtained from four different habitats (simple storage, open storage, tenemental storage, and alcoholizing storage) of tobacco storage, and mites were collected, isolated, identified, counted, and analyzed. Results Twenty-three species of mites belonging to 16 genera and 5 families were collected. The species number, species richness, and species diversity of mites were the highest in simple storage, followed by open storage, tenemental storage, and alcoholizing storage. The species evenness of mite community was the highest in simple storage (J'= 0.959), while the species domination of mite community was the highest in alcoholizing storage (D=0.150). The Jaccard similarity index between the mite communities in open storage and alcoholizing storage was the lowest (Q=0.238). Conclusion The community structure and diversity of mites breeding in tobacco storage in Anhui province are closely related to the habitat conditions, and there are relatively few mites breeding in alcoholizing storage.

【Abstract】 Objective To study the composition and diversity of Acaroid mites in stored produces in Xuancheng area. Methods Acaroid mites were isolated by light and worked out specimens to classify. Then the data was counted and analyzed. Results A total of 19 species of Acairdida were detected from 27 samples, belonging to 16 genera and 7 families. The average breeding density of the Mites was 131.63/g, and the species richness index was 1.72. The diversity index was 2.84, and the evenness index was 0.97. Conclusion Stored products in Xuancheng area were seriously polluted by acaroid mites, and the constitution of Acaridida community took on diversification. So it’s important to prevent acaroid mites and guard against human acarisis.

【Abstract】 Objective To develop a direct and effective method to screen drug that can kill human Demodex in vitro.  Methods Several methods including self-established method named Tracking-Detection were used in anti-mite experiments in vitro with Radix Stemonae alcohol extract individually. Way of drug delivery and anti-mite effect of all methods were evaluated.  Results All the traditional methods showed advantages and disadvantages in drug delivery and evaluation of anti?mite effect. Tracking-Detection was easy to be conducted with clear visual field, and the exact death time of each mite could be detected and the death time was (3.53±1.04) min with Radix stemonae alcohol extract. And then the differences of the anti-mite activities to human demodex of different drugs could be compared statistically by t-test. Conclusion Tracking-Detection is an ideal method to screen anti-mite drug killing human Demodex in vitro.